antibody against ocn  (Servicebio Inc)

Journal: Bioactive Materials

Article Title: Biomineralization-inspired scaffolds using citrate-based polymers to stabilize amorphous calcium phosphate promote osteogenesis and angiogenesis for bone defect repair

doi: 10.1016/j.bioactmat.2025.10.016

Figure Lengend Snippet: Biocompatibility and osteogenic activity of POC-ACP scaffolds under different polymerization conditions in vitro . A) Schematic diagram of rBMSC cultured with different scaffolds conditioned medium for the evaluation of osteogenic activity. B) Live/Dead staining of rBMSCs cultured with different POC-ACP scaffolds conditioned medium (Scale bar = 200 μm). C) ALP staining of rBMSCs cultured with different POC-ACP scaffolds conditioned medium at day 7 (Scale bar = 5 mm and 200 μm, respectively). D) ARS staining of rBMSCs cultured with different POC-ACP scaffolds conditioned medium at day 14 (Scale bar = 5 mm and 200 μm, respectively). E) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured in different POC-ACP scaffolds-conditioned medium (n = 3). F) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured in different POC-ACP scaffolds-conditioned medium. G) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). H) Schematic diagram of rBMSC cultured on different scaffolds for the evaluation of osteogenic activity. I) SEM images of rBMSCs cultured on different POC-ACP scaffolds (Scale bar = 20 μm). J) ALP staining of rBMSCs cultured on different POC-ACP scaffolds at day 7 (Scale bar = 1 cm). K) ARS staining of rBMSCs cultured on different POC-ACP scaffolds at day 14 (Scale bar = 1 cm). L) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured on different POC-ACP scaffolds (n = 3). M) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured on different POC-ACP scaffolds. N) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). Error bars, mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: IHC staining was performed on femur sections μm thick with primary antibodies against OCN (1:200, Proteintech, China) and CD31 (1:200, Proteintech, China), according to the described protocol [ ].

Techniques: Activity Assay, In Vitro, Cell Culture, Staining, Expressing, Western Blot, Standard Deviation

Journal: Bioactive Materials

Article Title: Biomineralization-inspired scaffolds using citrate-based polymers to stabilize amorphous calcium phosphate promote osteogenesis and angiogenesis for bone defect repair

doi: 10.1016/j.bioactmat.2025.10.016

Figure Lengend Snippet: POC-ACP promoted osteogenic differentiation compared to POC-HA scaffolds in vitro . A) Schematic diagram of rBMSC cultured with different scaffolds conditioned medium for the evaluation of osteogenic activity. B) ALP staining of rBMSCs cultured with different conditioned medium at day 7 (Scale bar = 5 mm and 200 μm, respectively). C) ARS staining of rBMSCs cultured with different conditioned medium at day 14 (Scale bar = 5 mm and 200 μm, respectively). D) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured in different conditioned medium (n = 3). E) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured in different conditioned medium. F) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). G) Schematic diagram of rBMSC cultured on different scaffolds for the evaluation of osteogenic activity. H) ALP staining of rBMSCs cultured on different scaffolds at day 7 (Scale bar = 1 cm). I) ARS staining of rBMSCs cultured on different scaffolds at day 14 (Scale bar = 1 cm). J) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured on different scaffolds (n = 3). K) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured on different scaffolds. L) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). The POC-ACP group indicates the POC-ACP3 (80 °C, 3 d) group. Error bars, mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: IHC staining was performed on femur sections μm thick with primary antibodies against OCN (1:200, Proteintech, China) and CD31 (1:200, Proteintech, China), according to the described protocol [ ].

Techniques: In Vitro, Cell Culture, Activity Assay, Staining, Expressing, Western Blot, Standard Deviation

Journal: Bioactive Materials

Article Title: Biomineralization-inspired scaffolds using citrate-based polymers to stabilize amorphous calcium phosphate promote osteogenesis and angiogenesis for bone defect repair

doi: 10.1016/j.bioactmat.2025.10.016

Figure Lengend Snippet: Histological staining analysis in vivo . A) Histological staining of new bone tissue by HE and Masson staining (Scale bar = 100 μm and 200 μm, respectively). B) HE staining of heart, liver, spleen, lung and kidney tissues (Scale bar = 50 μm). C Immunohistochemical staining of osteogenic (OCN) and angiogenic (CD31) in new bone tissue (Scale bar = 100 μm and 200 μm, respectively). D) Quantitative analysis of the number of OCN-positive cells (n = 3). E) Quantitative analysis of the number of CD31-positive cells (n = 3). The POC-ACP group indicates the POC-ACP3 (80 °C, 3 d) group. Error bars, mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: IHC staining was performed on femur sections μm thick with primary antibodies against OCN (1:200, Proteintech, China) and CD31 (1:200, Proteintech, China), according to the described protocol [ ].

Techniques: Staining, In Vivo, Immunohistochemical staining, Standard Deviation

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: miRNA-130b-3p upregulation impairs osteogenic differentiation in AIS patients by inhibiting the IGF1/ERK pathway

doi: 10.1007/s00018-025-05885-5

Figure Lengend Snippet: Impaired osteogenic differentiation potential of primary osteoblasts from AIS patients. a. left: Immunohistochemical staining of OPN in the facet joints of the spine from non-AIS control patients (up) and AIS patients (down). Mid: Alkaline Phosphatase staining of primary osteoblasts after 3 days of osteogenic differentiation from non-AIS control patients (up) and AIS patients (down). Right: Alizarin Red S staining of primary osteoblasts after 14 days of osteogenic differentiation from non-AIS control patients (up) and AIS patients (down) b. Quantification of immunohistochemical staining results ( n = 10) c. Quantification of Alkaline Phosphatase staining results ( n = 10) d. Quantification of Alizarin Red S staining results ( n = 10) e. Western blot (WB) results for primary osteoblasts from non-AIS control patients (left) and AIS patients (right) after 3, 7, and 14 days of osteogenic differentiation ( n = 4) f. Quantification of RUNX2 protein expression g. Quantification of OCN protein expression **: p <0.01; ***: p <0.001

Article Snippet: At RT, fixed cells were blocked with 5% bovine serum albumin (BSA) and 0.3% Triton X-100 for 30 min, followed by overnight incubation with the primary antibody against OCN (ProteinTech, Wuhan, China) at 4 °C.

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: miRNA-130b-3p upregulation impairs osteogenic differentiation in AIS patients by inhibiting the IGF1/ERK pathway

doi: 10.1007/s00018-025-05885-5

Figure Lengend Snippet: Overexpression of miR-130b-3p reduces osteogenic ability in primary osteoblasts from non-AIS control patients. a. RNA expression level of miR-130b in the overexpression group is elevated ( n = 3,) b. OCN mRNA expression level is reduced in the miR-130b overexpression group ( n = 8) c. RUNX2 expression level is reduced in the miR-130b overexpression group ( n = 3) d. OPN mRNA expression level is reduced in the miR-130b overexpression group ( n = 3) e. Western blot (WB) analysis showing osteogenic marker expression after miR-130b overexpression f. RUNX2 protein expression level is reduced in the miR-130b overexpression group ( n = 3) g. OPN protein expression level is reduced in the miR-130b overexpression group ( n = 3) h. Immunofluorescence staining of OCN in osteoblasts from the control group (top) and the miR-130b-3p mimic group (bottom) i. Alizarin red s staining after 14 days of osteogenic differentiation in the miR-130b-3p mimic group j. Statistical analysis of alizarin red s staining results ( n = 5) k. Alkaline phosphatase staining after 7 days of osteogenic differentiation in the miR-130b-3p mimic group l. Statistical analysis of alkaline phosphatase staining results ( n = 5) *: p <0.05

Article Snippet: At RT, fixed cells were blocked with 5% bovine serum albumin (BSA) and 0.3% Triton X-100 for 30 min, followed by overnight incubation with the primary antibody against OCN (ProteinTech, Wuhan, China) at 4 °C.

Techniques: Over Expression, Control, RNA Expression, Expressing, Western Blot, Marker, Immunofluorescence, Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: miRNA-130b-3p upregulation impairs osteogenic differentiation in AIS patients by inhibiting the IGF1/ERK pathway

doi: 10.1007/s00018-025-05885-5

Figure Lengend Snippet: miRNA-130b-3p overexpression inhibits osteogenic capacity of AIS patient primary osteoblasts through the IGF1/MAPK pathway. a. WB bands from AIS patient primary osteoblasts treated with NC Si, empty vector SiRNA; 130b Si, miRNA-130b-3p SiRNA; AXL1717, Picropodophyllin ( n = 3) b. IGF1 expression levels are elevated in the 130b Si group and 130b Si + AXL1717 group c. IGF1R expression levels show no significant changes in the 130b Si group and 130b Si + AXL1717 group d. P-IGF1R expression levels are elevated in the 130b Si group e. Phosphorylation of ERK1/2 is elevated in the 130b Si group f. IGF1R expression levels remain unchanged in the 130b Si and 130b Si + AXL1717 groups g. RUNX2 expression levels are elevated in the 130b Si group h. OCN expression levels are elevated in the 130b Si group i. Osteogenic differentiation of primary osteoblasts in the 130b Si group shows improved osteogenic capacity j. Quantification of average optical density of Alizarin Red S staining( n = 3) ***: p <0.001; **: p <0.01; *: p <0.05

Article Snippet: At RT, fixed cells were blocked with 5% bovine serum albumin (BSA) and 0.3% Triton X-100 for 30 min, followed by overnight incubation with the primary antibody against OCN (ProteinTech, Wuhan, China) at 4 °C.

Techniques: Over Expression, Plasmid Preparation, Expressing, Phospho-proteomics, Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: miRNA-130b-3p upregulation impairs osteogenic differentiation in AIS patients by inhibiting the IGF1/ERK pathway

doi: 10.1007/s00018-025-05885-5

Figure Lengend Snippet: miRNA-130b-3p overexpression causes impaired osteogenesis and axial curvature deformity in zebrafish. a. Development of zebrafish embryos after microinjection of miRNA-130b-3p mimic ( n = 100) b. Imaging of zebrafish with axial curvature deformity c. Alizarin Red S staining shows delayed vertebral development in zebrafish after miRNA-130b-3p overexpression d. Statistical analysis of vertebral development in zebrafish at day 7( n = 10) e. Expression levels of dre-miR-130b in zebrafish after miRNA-130b-3p mimic microinjection are elevated( n = 5) f. Expression levels of igf1 in zebrafish decrease after miRNA-130b-3p mimic microinjection( n = 5) g. Expression levels of col1a2 in zebrafish decrease after miRNA-130b-3p mimic microinjection( n = 5) h. Expression levels of ocn in zebrafish decrease after miRNA-130b-3p mimic microinjection( n = 5) ***: p <0.001; **: p <0.01; *: p <0.05

Article Snippet: At RT, fixed cells were blocked with 5% bovine serum albumin (BSA) and 0.3% Triton X-100 for 30 min, followed by overnight incubation with the primary antibody against OCN (ProteinTech, Wuhan, China) at 4 °C.

Techniques: Over Expression, Microinjection, Imaging, Staining, Expressing